Enzymes Used In DNA Replication

Different Enzymes and their role in DNA replication.

Helicase- an enzyme that unwinds and separates the two strands of DNA double helix in order to
               provide a single-stranded DNA for replication. The helicase moves down the line of DNA        
               and continues to separate the two strands throughout the replication process.

DNA Polymerase III- enzyme involved in prokaryotic DNA replication. For example the replication of
                                   bacteria.

DNA Polymerase I- enzyme that removes RNA primer from the lagging strand and fills the correct
                                necessary nucleotides fragments in the 5' to 3' direction. It proofreads for mistakes.

RNA Primase- an enzyme that synthesizes the RNA primer. It lays down a strand of 8-10 nucleotides
                        that is complimentary to the replicating strand of DNA. DNA polymerase cannot initiate
                        the synthesis of a DNA strand without an initial RNA primer.

Ligase- enzyme that joins the various fragments together into a continuous strand of DNA.